Reporter
Part:BBa_K1997004:Design
Designed by: Xinyuan Qiu Group: iGEM16_NUDT_CHINA (2016-10-13)
sGFP-N->Zif268->sGFP-C
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 604
Illegal AgeI site found at 688
Illegal AgeI site found at 772 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 775
Illegal BsaI.rc site found at 508
Illegal BsaI.rc site found at 989
Design Notes
This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds.
Source
This part is custom synthesized, the original sequence was acquired form fire fly